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目的探讨重组人内皮抑素(recombinanthumanendostatin, rhES)与5氟脲嘧啶(5 fluorouracil, 5 FU)联合应用对胃癌裸鼠移植瘤生长的抑制作用。方法建立胃癌异位移植BALB/C裸鼠模型,分为4组,每组6只。分别注射生理盐水,腹腔内注射5 FU(10mg/kg),rhES组瘤周注射rhES(2mg/kg)同时给予5 FU与rhES,每天1次,连用10d。计算肿瘤体积、抑瘤率及肿瘤缩小率,检测肿瘤组织血管内皮生长因子(VEGF)、碱性成纤维生长因子(bFGF)、血管内皮生长因子C(VEGF C)、Ⅷ因子相关抗原(FⅧAg)、增殖细胞核抗原(PCNA)、bcl 2表达及肿瘤细胞凋亡指数(AI)。结果rhES+5 FU组肿瘤体积为(43±2)mm3, 5 FU组为(169±45)mm3, rhES组为(95±28)mm3,对照组为(1057±114)mm3 (P<0 01 )。抑瘤率为99 6%,肿瘤缩小率为98 2%。用药前rhES+5 FU组肿瘤体积为( 207±50 )mm3,比5 FU组与rhES组下降更迅速(P<0 01 )。rhES+5 FU组与5 FU组VEGF、bFGF及VEGF C表达强度均为0 ~+;rhES+5 FU组PCNA及bcl 2表达最弱;rhES+5 FU组AI为11 7±1 1, 5 FU组为6 2±0 6,rhES组为5 8±0 8,对照组为2 4±0 6(P<0 01)。微血管密度在rhES+5 FU组为8 9±2 5,rhES组为10 0±1 5,均低于5 FU组(27 3±1 7)与对照组(29 9±2 3) (P<0 01 )。结论联合应用5 FU及rhES能显著抑制胃癌?
Objective To investigate the inhibitory effect of recombinant human interleukin (rhES) combined with 5 fluorouracil (5 FU) on the growth of transplanted gastric cancer in nude mice. Methods BALB / C nude mice models of gastric cancer were established. They were divided into 4 groups with 6 in each. Rats were injected with 5 FU (10 mg / kg) intraperitoneally and rhFS (2 mg / kg) given concurrently with 5 FU and rhES once daily for 10 days. The tumor volume, tumor inhibition rate and tumor shrinkage rate were calculated. The expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor C (VEGF C), factor Ⅷ related antigen (FⅧAg) , Proliferating cell nuclear antigen (PCNA), bcl 2 expression and tumor cell apoptosis index (AI). Results The tumor volume of the rhES + 5 FU group was (43 ± 2) mm3, that of the 5 FU group was (169 ± 45) mm3, that of the rhES + 5 FU group was (95 ± 28) mm3, and that of the control group was (1057 ± 114) mm3 01). The tumor inhibition rate was 99.6% and the tumor reduction rate was 98.2%. The tumor volume of rhES + 5 FU group was (207 ± 50) mm3 before treatment, which was more rapid than that of 5 FU group and rhES group (P <0.01). The expressions of VEGF, bFGF and VEGF C in rhES + 5 FU group and 5 FU group were all 0 ~ +. The expression of PCNA and bcl 2 in rhES + 5 FU group was the weakest. The AI in rhES + 5 FU group was 11 7 ± 1 1, 5 6 2 ± 0 6 in the FU group, 58 ± 0 8 in the rhES group, and 24 ± 0 6 in the control group (P <0.01). Microvessel density was 89 ± 25 in rhES + 5 FU group and 100 ± 1 5 in rhES group, both of which were lower than those in 5 FU group (27 3 ± 1 7) and control group (29 9 ± 2 3) (P < 0 01). Conclusion Combination of 5 FU and rhES can significantly inhibit gastric cancer?