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通常消化的方法有在氧等离子体中温灰化,高温灰化以及在酸中低温湿消化。但这些方法均不完善。在氧等离子体中低温灰化的设备昂贵。且多数灰化器一次只能处理少量样品。另外当盐含量高时,低温灰化很费时间。据报道在高温灰化时,一些无机物由于挥发或吸附在器壁而损失以及来自灰化器的污染。当要彻底消化样品中的脂类时,广泛采用用高氯酸湿法消化,还有产生爆炸的可能性。湿法消化时,有时需用大量的酸并要求操作者密切监视。在湿法消化中大量使用硫酸,特别是分析钙时干扰了原子吸收分析。本文所述的方法对七种生物学上的重要元素不产生损失且所需时间少,使用试剂少而降低了污染和空白。能更准确地分析低含量的样品。
Common methods of digestion include temperature ashing in oxygen plasma, ashing at high temperatures, and wet digestion at low temperatures in acid. However, these methods are not perfect. Low-temperature ashing in oxygen plasma is expensive. Most asher and can handle only a small number of samples. In addition, when the salt content is high, low temperature ashing takes a long time. Some inorganics are reported to lose due to volatilization or adsorption on the walls and contamination from the asher at high temperature ashing. When it comes to completely digesting the lipids in the sample, it is widely used to digest wet with perchloric acid and to explode. Wet digestion, sometimes need to use a lot of acid and require close monitoring of the operator. Sulfuric acid is widely used in wet digestion, and in particular, analysis of calcium interferes with atomic absorption analysis. The methods described herein do not result in loss of the seven biologically important elements and require less time and use less reagents to reduce contamination and void. Can analyze samples with low content more accurately.