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以Ｒ１０００，１５８３４，１８５５，８１９６等４种发根农杆菌菌株，在不同条件下，感染地不容（ＳｔｅｐｈａｎｉａｄｅｌａｖａｙｉＤｉｅｌｓ），结果在光照条件下，Ｒ１０００诱导植物幼嫩组织产生毛状根。毛状根在ＭＳ液体培养基中生长４２ｄ后，趋于稳定；异喹啉生物碱含量在第２８ｄ时达到峰值１１０４μｇ／ｇＦＷ。６种培养基中，毛状根生长及异喹啉生物碱含量的差异很大，在Ｂ５培养基中毛状根生长最快，Ｎｉｔｓｃｈ培养基中毛状根的异喹啉生物碱含量最高。Ｂ５培养基中，苯丙氨酸抑制毛状根的生长及异喹啉生物碱合成。
R1000, 15834, 1855, 8196 and other four kinds of Agrobacterium rhizogenes strains, under different conditions, not infected (StephaniadelavayiDiels), the results under light conditions, R1000 induced young plant hairy roots. The hairy roots tended to be stable after 42 days of growth in MS liquid medium. The content of isoquinoline alkaloids peaked at 1104 μg / g FW on the 28th day. The hairy root growth and isoquinoline alkaloid content varied greatly among the six kinds of media, and the hairy root grew fastest in B5 medium. The content of isoquinoline alkaloids in hairy roots was the highest in Nitsch medium. Phenylalanine inhibited the growth of hairy roots and the synthesis of isoquinoline alkaloids in B5 medium.