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本文首次报道用组织化学、免疫组织化学和免疫电泳等方法,研究东乡伊蚊自殖株卵黄发生过程中脂肪体合成卵黄原蛋白及其动态和血淋巴内卵黄原蛋白含量动态的实验结果。与同种蚊虫非自殖株的实验结果对比,发现自殖株蚊虫脂肪体细胞合成卵黄原蛋白在羽化后即启动,羽化初脂肪体细胞质内有密集的卵黄原蛋白颗粒,合成高峰见于羽化后24h,至36h开始下降,60h之脂肪体细胞内这种蛋白颗粒很稀少;卵母细胞的变化情况与此相反,随着脂肪体细胞内蛋白颗粒密度的下降,卵母细胞内的卵黄蛋白颗粒密度增加,并逐步发育成熟。非自殖株蚊虫血食前脂肪体细胞和卵母细胞发育有明显的休止期,合成卵黄原蛋白始于血食后,并于24h达高峰:卵母细胞的情况与自殖株蚊虫相似。实验还证实,无脂肪体外组织合成卵黄原蛋白。自殖株蚊虫血淋巴内卵黄原蛋白在羽化初就有一定浓度,羽化后36h达高峰,随后即下降;有非殖株血淋巴内卵黄原蛋白浓度高峰在血食后24h。上述结果表明,东乡伊蚊同种不同生理株的卵黄发生虽其过程一致,但其生物学特征各有不同,在脂肪体细胞合成卵黄原蛋白的启动时间,合成能力及卵黄原蛋白在血淋巴中的动态等方面存在差异。
This article for the first time reported the results of histochemistry, immunohistochemistry and immunoelectrophoresis to study the dynamics of vitellogenin and its dynamics and the content of vitellogenin in hemolymph during the development of self-propagating yolk in Aedes aegypti. Compared with the experimental results of non-adventitious plants of the same species of mosquitoes, it was found that the vitellogenin from autotrophs of autotrophic mosquito was activated immediately after eclosion, with dense yolk protein particles in the early fat body cytoplasm. The peak of synthesis was found after eclosion 24h, to 36h began to decline, 60h fat cells in this protein particles is very rare; oocyte changes in contrast with the fat cells within the protein particle density decreased, the yolk protein granules in the oocytes Increased density, and gradually mature. Non-adventitious mosquitoes pre-hematopoietic fat cells and oocyte development had a significant resting period, the synthesis of vitellogenin began after blood feeding and peaked at 24: the situation of oocytes and autotrophic mosquitoes similar. Experiments also confirmed that fat-free tissue synthesis of yolk protein. The primordial mosquito hemolymph vitellogenin had a certain concentration at the beginning of eclosion and peaked at 36h after eclosion, then decreased, and the highest concentration of vitellogenin in the hemolymph of non-colonial plants was at 24 h after ingestion. The above results show that although the process of yolk production of the same species of Ae. Albopictus is the same, its biological characteristics are different. The synthesis of yolk protein and the synthesis of yolk protein in the hemolymph In the dynamic and so there are differences.