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目的:建立同时测定知母药材中新芒果苷和芒果苷含量的方法,并测定不同产地知母中新芒果苷和芒果苷的含量,评价不同产地知母药材的质量。方法:采用超高效液相色谱(UPLC)法。色谱柱为ACQUITYUPLCBEHC18(100mm×2.1mm,1.7μm),流动相为0.4%冰醋酸水-甲醇(梯度洗脱),流速为0.25ml/min,柱温为35℃,检测波长为258nm。结果:新芒果苷和芒果苷的进样量分别在0.0300～0.4800、0.0285～0.4560μg范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999);精密度、重复性、稳定性试验的RSD均<3%;平均加样回收率分别为97.67%、100.17%,RSD分别为2.87%、2.78%(n均为6)。结论:该方法快速、准确,可用于同时测定知母中新芒果苷和芒果苷的含量。不同产地的知母质量存在差异。
OBJECTIVE: To establish a method for the simultaneous determination of ne mangiferin and mangiferin in Anemarrhena asphodeloides, and to determine the content of ne mangiferin and mangiferin in Anemarrhena asphodeloides from different areas and to evaluate the quality of Zhimu medicinal materials in different areas. Methods: The ultra-high performance liquid chromatography (UPLC) method. The chromatographic column was ACQUITY UPLC HBE 18 (100 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of 0.4% glacial acetic acid in water (gradient elution) with a flow rate of 0.25 ml / min and a column temperature of 35 ° C. The detection wavelength was 258 nm. Results: The injection volume of ne mangiferin and mangiferin showed a good linear relationship with the integral values of their peak areas (r = 0.9999) in the range of 0.0300 ~ 0.4800,0.0285 ~ 0.4560μg respectively. The precision, repeatability and stability The RSDs were all less than 3%. The average recoveries were 97.67% and 100.17%, respectively, with RSDs of 2.87% and 2.78%, respectively (n = 6). Conclusion: The method is rapid and accurate and can be used for the simultaneous determination of ne mangiferin and mangiferin in. There are differences in the quality of Anemarrhena between different areas.