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本文用已确定空间结构的溶菌酶为对象,采用可同时探测其主、侧链各基团变化的激光喇曼光谱技术,研究了YHPD对蛋白质空间结构的微观光敏损伤,获得以下新结果:(1)除5种已经发表的氨基酸(Trp,Tyr,Met,1/2 Cys和 His)外,还有苯丙氨酸和胱氨酸亦受到了光敏损伤;(2)定出氨基酸各基团对光敏作用敏感的次序;(3)被“埋藏”在蛋白质三维结构内部的色氨酸和酪氨酸也受到较强的损伤;(4)蛋白质的主链构象亦发生了明显的变化,有序结构(α-螺旋、β-折叠、β-回折)减少,无规卷曲增加.
In this paper, Lysozyme has been identified as the object of space structure, using simultaneous detection of the main and side groups of laser Raman spectroscopy changes in the microstructure of the protein YYPD microscopic photosensitive damage, the following new results: 1) In addition to the five published amino acids (Trp, Tyr, Met, 1/2 Cys and His), phenylalanine and cystine are also photo-damaged; (2) (3) the tryptophan and tyrosine buried in the three-dimensional structure of protein are also strongly damaged; (4) the main-chain conformation of the protein has also undergone significant changes, including Sequence structure (α-helix, β-sheet, β-sheet) decreases and random coil increases.