目的观察外源性直流电场对人视网膜色素上皮(hRPE)细胞移行行为的影响,揭示电场在RPE细胞损伤修复中的生理作用及临床意义。方法培养的hRPE细胞依培养液成分不同分为改良Eagle培养液(DMEM)组、DMEM+10%新生小牛血清(FBS)组,两组中未暴露于电场的细胞设为对照。将细胞分别暴露于强度为0、4、6、8V/cm的电场中,显微摄像系统连续记录2h中每隔15min细胞移行的变化图像,测量细胞移行距离及细胞移行方向与场线间夹角。图像分析系统处理结果。结果DMEM组中细胞对电场的反应较弱,电压加至6V/cm时可观察到细胞向阴极方向的移动趋势(不同电场强度中2h里所有单个细胞总体的位移方向指标之间,即平均cosineФ相比,P<0.05)。DMEM+10%FBS组中电场对细胞的作用加强,4V/cm时细胞出现长轴垂直于场线的排列方式,并向电场阴极运动,该现象随电场强度的增加而更为明显(不同电场强度之间及不同培养液之间平均cosineФ相比,P<0.05)。当电极方向转换后,细胞的定向移行方向随阴极方向而改变。结论外加电场可诱导hRPE细胞的电场阴极方向的定向移行,此作用受血清影响并与电场强度成正相关。在视网膜损伤后的早期修复过程中,内源性的电场可能是诱导hRPE细胞移行的始动因素之一。 Objective To observe the effect of exogenous DC electric field on the migration behavior of human retinal pigment epithelium (hRPE) cells, and to reveal the physiological role of electric field in the repair of RPE cells and its clinical significance. Methods The cultured hRPE cells were divided into modified Eagle’s medium (DMEM) and DMEM + 10% fetal bovine serum (FBS) groups according to the different components of the culture medium. Cells in the two groups that were not exposed to the electric field were set as controls. The cells were exposed to an electric field of 0, 4, 6 and 8 V / cm respectively. The microscopic imaging system continuously recorded the change of cell migration every 2 minutes in 2 hours, and measured the distance of migration and the direction of cell migration angle. Image Analysis System Processing Results. Results The cells in the DMEM group responded weakly to the electric field. When the voltage was increased to 6 V / cm, the tendency of the cells moving towards the cathode was observed (the average direction of displacement of all the single cells in 2 h in different electric field intensities Compared to P <0.05). In DMEM + 10% FBS group, the effect of electric field on cells was stronger. At 4V / cm, the long axis perpendicular to the field lines appeared in the cells and moved toward the cathode. This phenomenon was more obvious with the increase of electric field Intensity and between different culture medium average cosineФ compared, P <0.05). When the direction of the electrode is changed, the orientation direction of the cells changes with the direction of the cathode. CONCLUSION: The applied electric field can induce directional shift in the direction of electric field of hRPE cells, which is affected by serum and positively correlated with electric field intensity. During the early repair after retinal injury, the endogenous electric field may be one of the initiating factors that induce hRPE cell migration.