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目的：探讨无氧（Ｏ％Ｏ２＋９５％Ｎ２＋５％ＣＯ２）和低氧（２５～３％Ｏ２＋９２％Ｎ２＋５％ＣＯ２）对新生小牛肺动脉内皮细胞（ＰＡＥＣ）和肺动肺平滑肌细胞（ＰＡＳＭ）５－羟色胺转载体基因表达的影响。方法：应用细胞培养，核酸分子杂交技术。结果：无氧组和低氧组（１５ｈ、３ｈ、６ｈ）ＰＡＥＣ５－羟色胺转载体ｍＲＮＡ表达显著高于常氧组（Ｐ＜００５），而无氧和低氧１２ｈ至４８ｈ对ＰＡＥＣ５－羟色胺转载体ｍＲＮＡ表达无明显影响。无氧和低氧（３ｈ、６ｈ、１２ｈ、２４ｈ）组ＰＡＳＭ５－羟色胺ｍＲＮＡ表达均显著高于常氧组（Ｐ＜００１），结论：推测缺氧早期通过促进ＰＡＥＣ５－羟色胺转载体基因表达，加强ＰＡＥＣ对５－羟色胺的摄取和降解，随缺氧时间的延长，ＰＡＥＣ的此功能受损。缺氧ＰＡＳＭ５－羟色胺转载体基因表达的持续增加，则可能参与缺氧肺动脉高压的形成。
OBJECTIVE: To investigate the effects of oxygen free (O% O2 + 95% N2 + 5% CO2) and hypoxia (2.5% -3% O2 + 92% N2 + 5% CO2) on the proliferation of newborn calf pulmonary arterial endothelial cells (PAECs) and pulmonary artery smooth muscle cells Effect of serotonin transgene on gene expression. Methods: Cell culture and nucleic acid hybridization were used. Results: The mRNA expression of PAEC serotonin in anaerobic and hypoxic groups (15h, 3h, 6h) was significantly higher than that in normoxia group (P <005) - serotonin transcripts mRNA expression had no significant effect. PASM serotonin mRNA expression was significantly higher in hypoxia and hypoxia groups (P <0.01) at 3h, 6h, 12h and 24h. Conclusion: It is hypothesized that hypoxia could promote gene expression of serotonin receptor , To enhance PAEC serotonin uptake and degradation, with hypoxia time, PAEC this impaired function. The hypoxia PASM serotonin gene expression continues to increase, it may be involved in the formation of hypoxic pulmonary hypertension.