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本组用此法处理耳血涂片后在光镜下观察T、B淋巴细胞的百分比值,观察肿瘤及非肿瘤患者的细胞免疫状态,并用E-玫瑰花结试验,淋巴细胞转化试验进行对比。 T淋巴细胞染色后为园形、胞浆少、胞核为绿色,核大而园,胞浆边缘可见1个或数个染色颗粒,红色或棕红色者为酯酶阳性。pH不同时,色彩有变化。B淋巴细胞为20～40％,观察对象是经病理确诊的恶性肿瘤患者,其中原发性肺癌5例,鼻咽癌4例,食管癌2例,胃癌2例,淋巴肉瘤1例,直肠癌1例,
The group used this method to treat ear blood smears and observe the percentage of T and B lymphocytes under light microscope to observe the cellular immune status of tumor and non-tumor patients, and compare them with E-rose rosette test and lymphocyte transformation test. . The T lymphocytes were stained with round shape, few cytoplasm, and green nuclei. The nuclei were large and round. There were one or several stained granules on the cytoplasmic edge, and those with red or brown red were esterase positive. When the pH is different, the color changes. 20 to 40% of B-lymphocytes were observed in pathologically confirmed malignant tumors, including 5 primary lung cancers, 4 nasopharyngeal carcinomas, 2 esophageal cancers, 2 gastric cancers, 1 lymphosarcoma, and rectal cancer. In one case,