论文部分内容阅读
目的对桂郁金中的有效成分吉马酮进行含量测定,为科学评价和有效控制桂郁金的质量提供实验依据。方法采用高效液相色谱法,色谱柱美国Welch XB-C18柱(5μm,4.6mm×250mm),流动相:乙腈-水(70:30),流速:1mL/min,柱温:35C,检测波长:210nm,进样量:5μL。结果桂郁金的线性范围为14.9~84.5μg/mL之间,平均回收率为102.8%。结论该方法稳定线好、可靠,可应用于桂郁金的吉马酮的含量测定。
Objective To determine the content of gemcitabine in Guiyujin, and to provide experimental evidence for the scientific evaluation and effective control of the quality of Guiyujin. Methods High performance liquid chromatography (HPLC) was used on column Welch XB-C18 (5μm, 4.6mm × 250mm). The mobile phase consisted of acetonitrile and water (70:30), the flow rate was 1mL / : 210 nm, injection volume: 5 μL. Results Guiyu gold linear range of 14.9 ~ 84.5μg / mL, the average recovery was 102.8%. Conclusion The method is stable and reliable, and can be applied to the determination of gemcitabine in Guiyujin.