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苹果栽培品种秋富１与Ｍ７、Ｍ２６、八棱海棠３种砧木“Ｔ”形芽接１、２年生苗的嫁接口部位徒手切片显微镜观察，结果表明，砧木、接芽产生的大量愈伤组织，有不同方向、不同程度的分化，沿砧木和接芽的原有形成层逐渐分化产生新的形成层，使砧术与接穗维管组织连通；接口附近及过渡区域内各种组织均表现异常，维管分子及纤维细胞短、粗、弯曲，上下排列不同轴，射线细胞所占比例大，伸长较短，直径大；矮化砧Ｍ２６的嫁接口内愈伤组织细胞层数最多，导管不同轴程度最高；乔化砧八棱海棠的嫁接口内愈伤组织细胞层数最少，导管不同轴程度较轻；半矮化砧Ｍ７介于二者之间。
The results showed that lots of callus produced by rootstocks and buds in the rootstocks of Qiufu 1, M7, M26 and Malus sieboldii cultivars with 1 year and 2 year old seedlings were observed with hand-sliced microscopy. There are different directions, different degrees of differentiation, rootstock and bud along the original formation of the gradual differentiation of the formation of a new formation layer, so that anvil and scion vasculature connectivity; interface near the transitional region and the various organizations are abnormal, Vascular cells and fibroblasts were short, thick, curved, arranged in different axes up and down, the proportion of the ray cells was large, the elongation was short, and the diameter was large. The dying anvil M26 had the most callus layers in the grafts, The highest degree of coaxiality; Qiao anvil Balengli grafted callus callus layer at least, the degree of catheter less coaxial; semi-dwarfing anvil M7 between the two.