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Background Corneal neovascular leakage can lead to edema and secondary scarring.Previous studies have shownthat pericytes play a key role in maturation of angiogenesis.The present studies investigate the relationship betweenvascular permeability and pericyte coverage of endothelial cells in rat corneal neovascular induced by alkali burns.Methods Corneal neovascular vessels induced by alkali burns was performed in Sprague-Dawley rats.Corneas wereexcised on 1,2,3,5,7 and 10 days after cauterization.The vascular permeability rate was measured by the Evans bluemethod.The microvessel pericyte coverage index(MPI)was applied to quantify the pericyte coverage through doubleimmunofluorescent staining of frozen sections of corneas with CD31 as the endothelial and α-smooth muscle actin(α-SMA)as the pericyte markers.The correlation between permeability rate and MPI was analyzed.Pericyte coveragewas confirmed ultrastructually using transmission electron microscopy.Results The vascular permeability rate was(1.14±0.17),(0.24±0.08),(0.29±0.16),(0.14±0.10),(0.09±0.06)and(0.05±0.04)μg·ml~(-1)·mm~(-2)respectively on 1,2,3,5,7 and 10 days after cauterization.The MPI was 0,16.07%,11.95%,43.84%,73.97% and 86.21% respectively at the above mentioned time points.The correlation coefficientbetween MPI and the permeability rate was -0.943(P=0.005).Conclusions Pericyte recruitment was significantly correlated with the permeability of corneal neovascularizationinduced by alkali burns in rats.Therapeutic strategies aiming at anti-leakage should be most effective if they promotepericytes proliferation in the course of corneal neovascularization.Chin Med J 2007;120(4):274-279
Background Corneal neovascular leakage can lead to edema and secondary scarring. Previous studies have shownthat pericytes play a key role in maturation of angiogenesis. The present studies investigate the relationship between vascular permeability and pericyte coverage of endothelial cells in rat corneal neovascular induced by alkali burns. Methods Corneal neovascular vessels induced by alkali burns was performed in Sprague-Dawley rats. Corneas wereexcised on 1,2,3,5,7 and 10 days after cauterization. The vascular permeability rate was measured by the Evans blue method. The microvessel pericyte coverage index ( MPI) was applied to quantify the pericyte coverage through double immunofluorescent staining of frozen sections of corneas with CD31 as the endothelial and α-smooth muscle actin (α-SMA) as the pericyte markers. The correlation between permeability rate and MPI was analyzed. Pericyte coverage was confirmed ultrastructually using transmission electron microscopy. Results The vascular permeabilit y rate was (1.14 ± 0.17), (0.24 ± 0.08), (0.29 ± 0.16), (0.14 ± 0.10), (0.09 ± 0.06) and (0.05 ± 0.04) μg · ml -1 · mm ~ -2) respectively on 1,2,3,5,7 and 10 days after cauterization. The MPI was 0,16.07%, 11.95%, 43.84%, 73.97% and 86.21% at the above mentioned time points. Correlation coefficientbetween MPI and the permeability rate was -0.943 (P = 0.005) .Conclusions Pericyte recruitment was significantly correlated with the permeability of corneal neovascularization induced by alkali burns in rats. Therapeutic strategies aiming at anti-leakage should be most effective if they promote pericytes proliferation in the course of corneal neovascularization. Chin Med J 2007; 120 (4): 274-279