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目的:探讨子宫内膜整合素β3、细胞间隙连接蛋白43表达水平与体外受精-胚胎移植妊娠结局的关系,进而分析其与子宫内膜容受性的相关性。方法:前瞻性收集了IVF/ICSI-ET病例36例,其中11例临床妊娠者作为妊娠组,25例未妊娠者作为非妊娠组。于黄体中期取内膜,应用单克隆抗体免疫组化方法检测子宫内膜整合素β3和Cx43表达情况。结果:妊娠组与非妊娠组子宫内膜腔上皮整合素β3表达的HSCORE平均分数分别为0.55(0.00~1.60)和0.07(0.00~1.10),差异有显著性(P=0.02);而子宫内膜腺上皮整合素β3表达比较,两组之间差异无显著性;子宫内膜间质细胞和上皮细胞中Cx43表达在两组均未发现统计学差异。结论:子宫内膜腔上皮整合素β3表达水平可能与体外受精-胚胎移植妊娠结局有相关性。
Objective: To investigate the relationship between endometrial integrin β3, connexin 43 expression and pregnancy outcome of in vitro fertilization-embryo transfer, and to analyze the relationship between endometrial receptivity and endometriosis. Methods: Thirty-six patients with IVF / ICSI-ET were prospectively enrolled. Among them, 11 cases of clinical pregnancy were as pregnancy group and 25 cases of non-pregnancy as non-pregnancy group. Endometrium was obtained from the mid luteal phase. The expression of integrin β3 and Cx43 in the endometrium was detected by immunohistochemistry with monoclonal antibody. Results: The mean HSCORE expression of endometrial integrin β3 in pregnancy group and non-pregnancy group were 0.55 (0.00-1.60) and 0.07 (0.00-1.10), respectively, with significant difference (P = 0.02) The expression of integrin β3 was not significantly different between the two groups. Cx43 expression was not found between the two groups in endometrial stromal cells and epithelial cells. Conclusion: The expression of integrin β3 in endometrial luminal epithelium may be related to the pregnancy outcome of IVF-ET.