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目的探讨益髓生血颗粒含药血清对K562细胞向红系分化的影响。方法用SD大鼠制备益髓生血颗粒含药血清,分高、中、低和对照组,各组含药血清以20%终浓度添加到1640培养基中,并对K562细胞进行干预培养。采用联苯胺染色法观察各组血清干预K562细胞后,对蓝染细胞进行计数,计算细胞蓝染阳性率;采用实时荧光定量聚合酶链反应(real time polymerase chain reaction,RT-PCR)方法观察益髓生血颗粒干预K562细胞后珠蛋白基因mRNA表达情况。结果联苯胺染色结果显示高、中、低、对照组与空白组比较均能够显著提高K562细胞蓝染率(P<0.0001);与对照组比较,益髓生血颗粒含药血清高、中剂量组K562细胞蓝染数量提高(P<0.001)。RT-PCR结果显示益髓生血颗粒含药血清,高剂量组与对照组比较,能够促进α和γ-珠蛋白基因表达(P<0.05)。结论益髓生血颗粒含药血清能够促进K562细胞向红系分化。
Objective To investigate the effect of Yisui Shengxue granule-containing serum on erythroid differentiation of K562 cells. Methods The serum of Yisui Shengxue Granule was prepared in SD rats, divided into high, middle, low and control groups. The drug-containing serum of each group was added to 1640 medium at the final concentration of 20%, and K562 cells were cultured in intervention. After the K562 cells were treated with benzidine staining, the number of blue staining cells was counted and the ratio of blue staining was calculated. Real-time PCR was used to observe the effects of Effect of Sheng Sheng Xue Granule on mRNA Expression of Dilin in K562 Cells. Results The results of benzidine staining showed that the high, medium and low doses of control group and the control group were able to significantly increase the blue-dyeing rate of K562 cells (P <0.0001). Compared with the control group, K562 cells increased the amount of blue dye (P <0.001). The results of RT-PCR showed that the Yisui Shengxue granule containing serum could promote the gene expression of α and γ-globin (P <0.05) compared with the control group. Conclusion Yiyao Shengxue granule containing serum can promote the erythroid differentiation of K562 cells.