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目的: 研究日本血吸虫大陆株副肌球蛋白全基因核酸疫苗免疫C57 BL/6 及BALB/c 小鼠诱生的保护性免疫力。方法: 将构建的日本血吸虫大陆株副肌球蛋白全基因核酸疫苗 (pCMV-SjC97) 经后腿胫前肌免疫C57BL/6及BALB/c小鼠, 共免疫3 次, 每次间隔3 w k。末次免疫后3 w k 以血吸虫尾蚴攻击感染, 6 wk 后计数成虫负荷及肝、脾、肠组织虫卵数。设不含SjC97 编码基因的空载体质粒免疫组为对照组。结果: pCMV-SjC97 免疫C57 BL/6 小鼠主要诱生IgG2a 和IgG2b 亚类, 而免疫BALB/c小鼠除诱生IgG2a 和IgG2b 亚类外, 还诱生IgG1。pCMV-SjC97 免疫C57BL/6 小鼠诱生较明显的减虫率 (35.5% ~41.1% ) 和减卵率 (肝、脾及肠组织减卵率分别为44.5% ~59.6% 、56.7% ~82.4% 及57.9% ), 而对BALB/c小鼠未诱生保护性。结论: pCMV-SjC97 核酸疫苗能对C57BL/6 小鼠诱生较明显保护性免疫力, 而对BALB/c 小鼠未诱生免疫保护力
Objective: To study the protective immunity of C57BL / 6 and BALB / c mice immunized with para-myosin whole-genome DNA vaccine of Schistosoma japonicum. Methods: The constructed CMV DNA vaccine (pCMV-SjC97) was immunized C57BL / 6 and BALB / c mice via tibialis anterior tibialis three times with 3 wk intervals . 3 wk after the last immunization challenge with Schistosoma japonicum cercariae infection, 6 wk after the count and adult worms liver, spleen, intestinal tissue number of eggs. The empty vector plasmid containing SjC97 coding gene was used as the control group. RESULTS: Immunization of BALB / c mice with pCMV-SjC97-immunized C57 BL / 6 mice induced mainly IgG2a and IgG2b subclasses, whereas IgG1 was induced in immunized BALB / c mice in addition to the induction of IgG2a and IgG2b subclasses. The induced worm reduction rate (35.5% ~ 41.1%) and the rate of egg reduction (the reduction rates of liver, spleen and intestine were 44.5% ~ 59 in pCMV-SjC97-immunized C57BL / .6%, 56.7% ~ 82.4% and 57.9% respectively), while no protection was induced in BALB / c mice. Conclusion: The pCMV-SjC97 DNA vaccine can induce more protective immunity in C57BL / 6 mice and no immunoprotective effect in BALB / c mice