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目的： 研究日本血吸虫大陆株副肌球蛋白全基因核酸疫苗免疫Ｃ５７ ＢＬ／６ 及ＢＡＬＢ／ｃ 小鼠诱生的保护性免疫力。方法： 将构建的日本血吸虫大陆株副肌球蛋白全基因核酸疫苗 （ｐＣＭＶ－ＳｊＣ９７） 经后腿胫前肌免疫Ｃ５７ＢＬ／６及ＢＡＬＢ／ｃ小鼠， 共免疫３ 次， 每次间隔３ ｗ ｋ。末次免疫后３ ｗ ｋ 以血吸虫尾蚴攻击感染， ６ ｗｋ 后计数成虫负荷及肝、脾、肠组织虫卵数。设不含ＳｊＣ９７ 编码基因的空载体质粒免疫组为对照组。结果： ｐＣＭＶ－ＳｊＣ９７ 免疫Ｃ５７ ＢＬ／６ 小鼠主要诱生ＩｇＧ２ａ 和ＩｇＧ２ｂ 亚类， 而免疫ＢＡＬＢ／ｃ小鼠除诱生ＩｇＧ２ａ 和ＩｇＧ２ｂ 亚类外， 还诱生ＩｇＧ１。ｐＣＭＶ－ＳｊＣ９７ 免疫Ｃ５７ＢＬ／６ 小鼠诱生较明显的减虫率 （３５．５％ ～４１．１％ ） 和减卵率 （肝、脾及肠组织减卵率分别为４４．５％ ～５９．６％ 、５６．７％ ～８２．４％ 及５７．９％ ）， 而对ＢＡＬＢ／ｃ小鼠未诱生保护性。结论： ｐＣＭＶ－ＳｊＣ９７ 核酸疫苗能对Ｃ５７ＢＬ／６ 小鼠诱生较明显保护性免疫力， 而对ＢＡＬＢ／ｃ 小鼠未诱生免疫保护力
Objective: To study the protective immunity of C57BL / 6 and BALB / c mice immunized with para-myosin whole-genome DNA vaccine of Schistosoma japonicum. Methods: The constructed CMV DNA vaccine (pCMV-SjC97) was immunized C57BL / 6 and BALB / c mice via tibialis anterior tibialis three times with 3 wk intervals . 3 wk after the last immunization challenge with Schistosoma japonicum cercariae infection, 6 wk after the count and adult worms liver, spleen, intestinal tissue number of eggs. The empty vector plasmid containing SjC97 coding gene was used as the control group. RESULTS: Immunization of BALB / c mice with pCMV-SjC97-immunized C57 BL / 6 mice induced mainly IgG2a and IgG2b subclasses, whereas IgG1 was induced in immunized BALB / c mice in addition to the induction of IgG2a and IgG2b subclasses. The induced worm reduction rate (35.5% ~ 41.1%) and the rate of egg reduction (the reduction rates of liver, spleen and intestine were 44.5% ~ 59 in pCMV-SjC97-immunized C57BL / .6%, 56.7% ~ 82.4% and 57.9% respectively), while no protection was induced in BALB / c mice. Conclusion: The pCMV-SjC97 DNA vaccine can induce more protective immunity in C57BL / 6 mice and no immunoprotective effect in BALB / c mice