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目的:探讨冠心病患者外周血中肝细胞生长因子浓度与CD133+内皮祖细胞数目的关系及临床意义。方法:30例冠心病患者(冠心病组)与30例非冠心病患者(非冠心病组),采用密度梯度离心法获取外周血单个核细胞,以FITC-鼠抗人CD133标记,流式细胞仪检测其CD133+细胞数目;用ELISA法检测血浆中肝细胞生长因子浓度。结果:与非冠心病组比较,冠心病组外周血中CD133+细胞数目少,血浆中肝细胞生长因子浓度高,差异均有统计学意义(P<0.01)。冠心病患者外周血中CD133+细胞数量与肝细胞生长因子浓度呈负相关。结论:检测外周血中CD133+细胞和血浆中肝细胞生长因子浓度,有望成为诊断冠心病的辅助检查。
Objective: To investigate the relationship between the concentration of hepatocyte growth factor (HGF) and the number of endothelial progenitor cells (CD133 +) in peripheral blood of patients with coronary heart disease and its clinical significance. Methods: Peripheral blood mononuclear cells were obtained from 30 patients with coronary heart disease (CHD) and 30 patients with non-CHD (non-CHD) by density gradient centrifugation. FITC-anti-human CD133, The number of CD133 + cells was detected. The concentration of hepatocyte growth factor in plasma was detected by ELISA. Results: Compared with non-coronary heart disease group, the number of CD133 + cells and the concentration of hepatocyte growth factor in coronary heart disease group were significantly lower than those of non-coronary heart disease group (P <0.01). The number of CD133 + cells in peripheral blood of patients with coronary heart disease was negatively correlated with the concentration of hepatocyte growth factor. Conclusion: Detection of CD133 + cells and plasma concentrations of hepatocyte growth factor in peripheral blood is expected to be an auxiliary examination for the diagnosis of coronary heart disease.