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目的 :探讨大鼠卵巢组织异体异位移植后的生长发育及功能情况。方法 :将成年大鼠卵巢组织植入去势成年雌性大鼠的肾被膜下 ,观察动情周期的恢复及维持 ,通过组织学和组织化学研究移植效果和测定血清雌激素水平观察卵巢内分泌功能。结果 :移植后 13 55± 2 81d出现动情周期 ,对移植卵巢的组织学观察可见不同发育阶段的各级卵泡及间质腺 ;3- β -羟甾脱氢酶组织化学显示它们呈不同程度阳性反应 ;血清学研究显示移植卵巢具有与正常卵巢相近的分泌雌激素的能力。结论 :大鼠卵巢异体异位移植能存活、发育并具有内分泌功能
Objective: To investigate the growth and function of rat ovarian tissue after allogeneic transplantation. METHODS: Adult ovariectomized rats were implanted into the subrenal capsule of ovariectomized adult female rats. The recovery and maintenance of estrous cycle were observed. The ovarian endocrine function was observed by histological and histochemical staining and serum estrogen levels. RESULTS: The estrous cycle was observed at 13 55 ± 21 days after transplantation. The histological examination of the ovarian tissue at all stages revealed follicles and interstitial glands at different developmental stages. The histochemical examination of 3-β-hydroxysteroid dehydrogenase showed that they were positive in varying degrees Serological studies have shown that transplanted ovaries have the ability to secrete estrogen close to the normal ovary. Conclusion: Allograft xenograft in ovary can survive, develop and have endocrine function