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应用二次回归通用旋转组合设计,组建了影响叶螨磷酸酯酶(酸性和碱性)活性的四因子(缓冲液pH值X1、温浴时间X2、反应温度X3、底物浓度X4)数学模型:Y酸性=0456380+0107889X2+0069027X3-0026836X12-0030794X32,F=2498,P<001;Y碱性=0267286-0200736X1+0049541X2+0030930X3-0049063X1X2+0053585X12-0049665X22,F=5768,P<001。结果表明,温浴时间是影响叶螨酸性磷酸酯酶活性的关键因子,在缓冲液pH44、底物浓度85×10-3mol/L、42℃温浴40min测得该酶活性最强。影响碱性磷酸酯酶活性的关键因子则是缓冲液pH值,pH90、37℃恒温30min、底物75×10-3mol/L的条件下,光密度值最大。两种酶的最大吸收峰波长为405nm。
A four-factor (buffer pH value X1, incubation time X2, reaction temperature X3, substrate concentration X4) mathematical model affecting the activity of spider mite phosphatase (acidic and basic) was established by quadratic regression general rotation combination design. Y acidity = 0456380 + 0107889X2 + 0069027X3-0026836X12-0030794X32, F = 2498, P <001; Y basicity = 0267286-0200736X1 + 0049541X2 + 0030930X3-0049063X1X2 + 0053585X12-0049665X22, F = 5768, P <001. The results showed that the incubation time was the key factor influencing the acid phosphatase activity of spider mites. The enzyme activity was the strongest at pH44 buffer, substrate concentration 85 × 10-3 mol / L, temperature 40 ℃ for 40 min. The key factor affecting the activity of alkaline phosphatase is the buffer pH value, pH90, 37 ℃ constant temperature 30min, the substrate under the conditions of 75 × 10-3mol / L, the optical density value maximum. The maximum absorption peak wavelength of both enzymes is 405 nm.