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Cry1Ac杀虫蛋白基因在转cry1Ac/CpTI双价抗虫水稻(OryzasativaL.)各部位中均得到了表达。不同水稻生育期及不同部位中Cry1Ac杀虫蛋白的表达量均有所不同。总的趋势是生育前期的表达量较生育后期高。在时间上,主茎叶片中的杀虫蛋白在拔节期最高,孕穗期开始下降,在扬花期和和灌浆期达到最低,到成熟期略有回升;主茎中的杀虫蛋白在苗期、拔节期和扬花期较高,种子中含量很低。不同生育期转基因水稻主茎顶叶对二化螟(Chilosuppressalis)初孵幼虫表现出了很强的抗虫性。在灌浆期前,取食转基因水稻顶叶的二化螟幼虫校正死亡率均90%左右,灌浆期后抗虫性有所下降,校正死亡率在60%左右。活体成株测定法评价结果表明,转cry1Ac/CpTI双价抗虫水稻对二化螟表现高抗。
The Cry1Ac insecticidal protein gene was expressed in all parts of Cry1Ac / CpTI bivalent insect-resistant rice (Oryzasativa L.). Different rice growth stages and different parts of Cry1Ac insecticidal protein expression levels are different. The general trend is that the early stage of expression is higher than the late stage of childbirth. In time, the insecticidal proteins in the main stem leaves had the highest at jointing stage, decreased at booting stage, reached the lowest at flowering stage and filling stage, and rose slightly at maturity stage. , Jointing and flowering period is higher, the content of seeds is very low. The main stem parietal lobe of transgenic rice at different growth stages showed strong resistance to newly hatched larvae of Chilosuppressalis. Before the grain filling stage, the corrected mortality of the striped stem borer (Chilo suppressalis) larvae fed on the top leaves of transgenic rice was about 90%. The resistance to insects decreased after filling, and the corrected mortality rate was about 60%. The results of in vivo determination of adult plants showed that transgenic rice with cry1Ac / CpTI showed high resistance to Chilo suppressalis.