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本文旨在探讨急性强直电刺激右后背海马(hippocampus,HPC)诱导出现双侧皮层网络癫痫的前背HPC神经元电生理机制。实验共用雄性SD大鼠35只,急性强直电刺激(60Hz,2s,0.4-0.6mA)大鼠右侧后背HPC(a-cute tetanization of the right posterior dorsal hippocampus,ATPDH)诱发癫痫模型。四通道同步记录双侧皮层电图(electroeorticogram,ECoG)或前背HPC深部电图和双侧前背HPC单位放电。结果显示,ATPDH可以引起以下效应:(1)双侧皮层癫痫样电活动起源于同侧HPC单位后放电,继而引起对侧HPC单位后放电,最终形成对侧和同侧皮层电图癫痫发作样电振荡;(2)增强双侧皮层4-10Hz节律性电振荡或诱发双侧HPC100-250Hz电振荡,与此同时双侧HPC神经元均表现为非对称性电活动;(3)双侧HPC神经元锋电位间期(interspike interval,ISI)点呈现非规则性环状分布。同侧HPC爆发式单位放电ISI点分布的环状分层较规则、发生率较高,并与同侧HPC电振荡最大振幅的正弦式波动形成明显的时间对应关系。结果表明:ATPDH可诱导双侧皮层和HPC电图形成癫痫电网络。当皮层或HPC癫痫网络重建过程中,HPC神经元放电具有特征性ISI点环状分布等编码形式。
This article aims to investigate the electrophysiological mechanism of acute tetanic stimulation of anterior dorsal HPC neurons induced by hippocampus (HPC) in bilateral cortical epilepsy. Thirty-five male Sprague-Dawley rats were used in this study. Acute tetanic stimulation (60 Hz, 2 s, 0.4-0.6 mA) induced a model of epilepsy induced by a-cute tetanization of the right posterior dorsal hippocampus (ATPDH). Four-channel simultaneous recordings of bilateral electrocorticogram (ECoG) or anterior HPC deep electrograms and bilateral anterior HPC discharges. The results showed that: ATPDH can cause the following effects: (1) bilateral cortical epileptiform electric activity originated in the ipsilateral HPC units after discharge, and then cause contralateral HPC unit discharge, the final formation of contralateral and ipsilateral cortical seizure-like (2) to enhance the bilateral cortex 4-10Hz rhythmic electrical oscillations or induce bilateral HPC 100-250Hz electrical oscillations, while bilateral HPC neurons showed asymmetric electrical activity; (3) bilateral HPC The neuronal interspike interval (ISI) showed an irregular ring distribution. The annular stratification of the ISI distribution in the ipsilateral HPC explosive unit discharge is more regular and has a higher incidence, and forms a significant time correspondence with the sinusoidal fluctuation of the maximum amplitude of the ipsilateral HPC oscillation. The results show that: ATPDH can induce bilateral cortical and HPC electrocephalus to form epileptiform network. When the cortex or HPC epilepsy network reconstruction process, HPC neuronal discharge has a characteristic ISI point ring distribution encoding.