论文部分内容阅读
通过建立鼠心腹腔异位移植模型研究人参二醇组皂甙（PDS）对缺血心肌再灌注损伤的保护作用及其浓度－效应关系。动物随机分为Ⅰ组（St·ThomasⅡ号液对照组）、Ⅱ组（加入PDS10mg／L组）、Ⅲ组（加入PDS20mg／L组）、Ⅳ组（加入PDS40mg／L组）、Ⅴ组（加入PDS80mg／L组）。结果：各组心肌丙二醛（MDA）和线粒体记分由大到小的顺序是Ⅴ＞Ⅰ＞Ⅱ＞Ⅲ＞Ⅳ，而各组心肌谷胱甘肽过氧化物酶（GSH－Px）活性由大到小的顺序是：Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ＞Ⅴ。Ⅳ组与其余各组相比有显著差异（P＜0．01）。结论：适宜浓度PDS作为外源性氧自由基清除剂对缺血心肌再灌注损伤有保护作用，其最适宜浓度为40mg／L。
To study the protective effect and the concentration-response relationship of Panaxadiol Saponins (PDS) on ischemia-reperfusion injury in rats by establishing rat heterotopic abdominal transplantation model. The animals were randomly divided into four groups: control group (St · ThomasⅡ), group Ⅱ (PDS10mg / L group), group Ⅲ (PDS20mg / L group), group Ⅳ (PDS40mg / L group) PDS80mg / L group). Results: The order of myocardial malondialdehyde (MDA) and mitochondria score was Ⅴ> Ⅰ> Ⅱ> Ⅲ> Ⅳ, while the activity of GSH-Px in each group was Big to small order is: Ⅳ> Ⅲ> Ⅱ> Ⅰ> Ⅴ. Ⅳ group compared with the other groups were significantly different (P <0.01). Conclusion: PDS as a protective agent against ischemia-reperfusion injury is a suitable concentration of PDS as its scavenger. Its optimal concentration is 40 mg / L.