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通过建立鼠心腹腔异位移植模型研究人参二醇组皂甙(PDS)对缺血心肌再灌注损伤的保护作用及其浓度-效应关系。动物随机分为Ⅰ组(St·ThomasⅡ号液对照组)、Ⅱ组(加入PDS10mg/L组)、Ⅲ组(加入PDS20mg/L组)、Ⅳ组(加入PDS40mg/L组)、Ⅴ组(加入PDS80mg/L组)。结果:各组心肌丙二醛(MDA)和线粒体记分由大到小的顺序是Ⅴ>Ⅰ>Ⅱ>Ⅲ>Ⅳ,而各组心肌谷胱甘肽过氧化物酶(GSH-Px)活性由大到小的顺序是:Ⅳ>Ⅲ>Ⅱ>Ⅰ>Ⅴ。Ⅳ组与其余各组相比有显著差异(P<0.01)。结论:适宜浓度PDS作为外源性氧自由基清除剂对缺血心肌再灌注损伤有保护作用,其最适宜浓度为40mg/L。
To study the protective effect and the concentration-response relationship of Panaxadiol Saponins (PDS) on ischemia-reperfusion injury in rats by establishing rat heterotopic abdominal transplantation model. The animals were randomly divided into four groups: control group (St · ThomasⅡ), group Ⅱ (PDS10mg / L group), group Ⅲ (PDS20mg / L group), group Ⅳ (PDS40mg / L group) PDS80mg / L group). Results: The order of myocardial malondialdehyde (MDA) and mitochondria score was Ⅴ> Ⅰ> Ⅱ> Ⅲ> Ⅳ, while the activity of GSH-Px in each group was Big to small order is: Ⅳ> Ⅲ> Ⅱ> Ⅰ> Ⅴ. Ⅳ group compared with the other groups were significantly different (P <0.01). Conclusion: PDS as a protective agent against ischemia-reperfusion injury is a suitable concentration of PDS as its scavenger. Its optimal concentration is 40 mg / L.