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目的研究ATP生物荧光技术快速测定细菌总数的应用。方法通过生物荧光检测系统与其配套的试剂盒对细菌悬液进行定量检测,同时与平板活菌计数培养法作平行比较。结果经对金黄色葡萄球菌、大肠杆菌和绿脓杆菌菌悬液检测结果显示,三种细菌菌悬液检出菌数的对数值与ATP值的对数值之间呈线性关系。金黄色葡萄球菌、大肠杆菌和绿脓杆菌回归方程依次为Y=1.209+0.744X(R2=0.942),Y=0.885+0.831X(R2=0.961)和Y=0.955+0.953X(R2=0.983)。ATP生物荧光法适用于检测含菌量<107cfu/ml的菌悬液。结论 ATP生物荧光法可快速地评价受细菌污染的液体中细菌总数,但溶液中细菌含量应控制在<107cfu/ml。
Objective To study the application of ATP biofluorescence assay to determine the total number of bacteria. Methods The bacterial suspension was quantitatively detected by the biofluorescence detection system and its accompanying kit, and compared with the viable plate count cultivation method. Results The detection results of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa suspension showed that there was a linear relationship between the logarithm of the number of bacteria detected by the three bacterial suspensions and the logarithm of ATP value. The regression equations of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were Y = 1.209 + 0.744X (R2 = 0.942), Y = 0.885 + 0.831X (R2 = 0.961) and Y = 0.955 + 0.953X (R2 = 0.983) . ATP biofluorescence method is suitable for the detection of bacterial suspension containing <107 cfu / ml of bacteria. Conclusion ATP biofluorescence can rapidly evaluate the total bacterial count in the liquid contaminated by bacteria, but the bacterial content in the solution should be controlled at <107 cfu / ml.