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目的探讨药物对大鼠睾丸缺血-再灌流后生精功能的影响。方法雄性SD大鼠32只,随机分成假手术组、扭转组、己酮可可碱组和维拉帕米组,每组8只,建立睾丸扭转动物模型。各组于复位前 15 min分别静脉注射生理盐水、己酮可可碱和维拉帕米,术后24 h留取手术侧睾丸。应用流式细胞术 (FCM)检测各组生精细胞凋亡和各级生精细胞计数,测定组织内丙二醛(MDA)含量、髓过氧化物酶 (MPO)和超氧化物歧化酶(SOD)活性。结果与扭转组相比,已酮可可碱组和维拉帕米组生精细胞凋亡明显减少,单倍体细胞群计数显著增多,超氧化物歧化酶活性回升,丙二醛含量和髓过氧化物酶活性下降, 其差异有显著性(P<0．05)。结论已酮可可碱和维拉帕米对睾丸缺血-再灌流后的生精能力具有明显的保护作用。
Objective To investigate the effect of drugs on the function of spermatogenesis in rats after testis ischemia-reperfusion. Methods Thirty-two male Sprague-Dawley rats were randomly divided into sham operation group, torsion group, pentoxifylline group and verapamil group, with 8 rats in each group. Animal model of testicular torsion was established. Rats in each group were injected with normal saline, pentoxifylline and verapamil intravenously 15 min before the procedure, and the operation side testis was taken 24 hours after the operation. Flow cytometry (FCM) was used to detect the apoptosis of spermatogenic cells and the number of spermatogenic cells in each group. The contents of malondialdehyde (MDA), myeloperoxidase (MPO) and superoxide dismutase SOD activity. Results Compared with the untreated group, the apoptosis of spermatogenic cells in pentoxifylline group and verapamil group was significantly decreased, the haploid population was significantly increased, the activity of superoxide dismutase (SOD), the content of malondialdehyde Oxidase activity decreased, the difference was significant (P <0.05). Conclusion Both pentoxifylline and verapamil have protective effects on spermatogenesis after testis ischemia-reperfusion.