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在已克隆出的刺五加鲨烯合酶基因(squalene synthase,ss)基因cDNA序列基础上,利用TAIL-PCR技术克隆SS的基因组DNA及启动子序列.克隆得到刺五加SS的DNA序列长8 244 bp,启动子序列长1 984 bp,转录起始位点位于起始密码子ATG上游568 bp处.基因包括13个外显子,12个内含子,其剪切符合GT-AG原则.启动子序列含有37个顺式作用元件,其中包括128个TATA-box、38个CAAT-box等核心调控元件,并且含有部分光响应元件、激素应答的元件等顺式调控元件.本研究首次克隆出刺五加SS基因组DNA及启动子序列,为后续研究SS基因表达的调控机制有很大帮助.“,”Based on the sequence of squalene synthase (SS) gene in Eleutherococcus senticosus,SS genomic and promoter sequence were cloned by using TAILPCR technique.The length of cloned SS gene DNA is 8 244 bp and the length of cloned promoter sequence is 1 984 bp,the transcription start site is located 568 bp in initiation codon ATG of the upper reaches.SS gene included 13 exon and 12 intron,and the splicing rules of its exon and intron were consistent with GT-AG.The promoter sequence contain 37 cis-regulatory elements,which contain core regulatory element such as 128 TATA-box and 38 CAAT-box,and it includes part of related to light response and hormone signal response.In this research,SS genomic DNA and promoter sequence was cloned from E.senticosus for the first time,it gives a hand for studying SS gene expression of regulation mechanism.