Micro-arc oxidized Cu-incorporated TiO_2 coatings(Cu—TiO_2) were prepared in the Ca,P,Cu-containing electrolyte to obtain an implant material with superior biological activity and antibacterial property.The surface topography,phase,and element composition of the TiO_2 and Cu—TiO_2 coatings were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD),and energy-dispersive X-ray spectrometry (EDS),respectively.Staphylococcus aureus(S.aureus) was selected to evaluate the antibacterial property of the Cu—TiO_2 coatings,whereas osteoblastic MG63 cells were cultured on the coatings to investigate the biological activity.The obtained results demonstrated that Cu element was successfully incorporated into the porous nano-structured TiO_2 coatings,which did not alter apparently the surface topography and phase composition of the coatings as compared to the Cu-free TiO_2 coatings.Moreover,the antibacterial studies suggested that the Cu-incorporated TiO_2 coatings could significantly inhibit the adhesion of S.aureus.In addition,the in vitro biological evaluation displayed that the adhesion,proliferation and differentiation of MG63 cells on the Cu-incorporated coatings were enhanced as compared to those on the Cu-free coatings and Ti plates.In conclusion,the innovative Cu-incorporated nano-structured TiO_2 coatings on Ti substrate with excellent antibacterial property and biological activity are promising candidates for orthopedic implant. Micro-arc oxidized Cu-incorporated TiO 2 coatings (Cu-TiO 2) were prepared in the Ca, P, Cu-containing electrolyte to obtain an implant material with superior biological activity and antibacterial property. The surface topography, phase, and element composition of the TiO 2 and Cu-TiO 2 coatings were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy-dispersive X-ray spectrometry (EDS), respectively.Staphylococcus aureus (S.aureus) was selected to evaluate the antibacterial property of the Cu-TiO 2 coatings, the osteoblastic MG63 cells were cultured on the coatings to investigate the biological activity. The obtained results demonstrated that Cu element was successfully incorporated into the porous nano-structured TiO 2 coatings, which did not alter apparently the surface topography and phase composition of the coatings as compared to the Cu-free TiO 2 coatings. More over, the antibacterial studies suggested that the Cu-incorporated TiO 2 coatings could si gnificantly inhibit the adhesion of S. aureus. addition, the in vitro biological evaluation displayed that the adhesion, proliferation and differentiation of MG 63 cells on the Cu-incorporated coatings were enhanced as compared to those on the Cu-free coatings and Ti plates. In conclusion, the innovative Cu-incorporated nano-structured TiO 2 coatings on Ti substrates with excellent antibacterial properties and biological activity are promising candidates for orthopedic implants.