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目的探讨酒精对大鼠睾丸诱导型一氧化氮合酶(iNOS)、Bcl-2基因表达和生精细胞凋亡的影响。方法30只成年健康SD雄性大鼠随机均分为对照组、低剂量组和高剂量组,用不同剂量的酒精灌胃成年大鼠26d(两个生精周期)后,免疫组织化学法(SP法)检测睾丸iNOS、Bcl-2基因表达的变化;原位缺口末端标记法(TUNEL法)检测细胞凋亡指数(A1)的变化。结果与对照组相比,低剂量组大鼠睾丸iNOS、Bcl-2基因表达强度和细胞凋亡指数(A1)无明显变化(P>0.05);而高剂量组与对照组和低剂量组相比,iNOS表达显著增强(P<0.01),Bcl-2基因表达明显减弱(P<0.01,P<0.05),细胞凋亡指数则增加(P<0.01)。结论长期大量饮酒可以诱导睾丸生精细胞凋亡增加,iNOS与Bcl-2基因表达的改变是重要原因之一。
Objective To investigate the effects of alcohol on the expression of iNOS, Bcl-2 gene and the apoptosis of spermatogenic cells in rats. Methods Thirty adult male Sprague-Dawley rats were randomly divided into control group, low dose group and high dose group. The rats were administered with different doses of alcohol for 26 days (two spermatogenic cycles) Method) to detect the changes of testis iNOS and Bcl-2 gene expression. The change of apoptosis index (A1) was detected by TUNEL method. Results Compared with the control group, the expression of iNOS and Bcl-2 in the testes of the low-dose group did not change significantly (P> 0.05); while the high-dose group and the control group and the low-dose group (P <0.01). The expression of Bcl-2 gene was significantly decreased (P <0.01, P <0.05) and the apoptosis index was increased (P <0.01). Conclusion Long-term heavy drinking can induce apoptosis of spermatogenic cells in testis. The change of iNOS and Bcl-2 gene expression is one of the important reasons.