论文部分内容阅读
采用单克隆抗体（单抗）和ＷＨＯ６７／９７标准血清建立ＥＬＩＳＡ法测定人血清免疫球蛋白Ｇ（ＩｇＧ）亚类浓度。结果表明：本法敏感（最低可测值为２．１ｎｇ／ｍｌ，可测范围为１００倍），精确（不同批测定平均变异系数≤８．０６％）。所用单抗特异性强，反应性好，ＩｇＧ亚类之和与常规单扩法所测总ＩｇＧ值密切相关（ｒ＝０．９６，Ｐ＜０．０１）．强调将单抗包被于经ＢＳＡ－戊二醛处理的微孔板上，能明显提高单抗吸附力和反应性；两种单抗混合包被可增加ＩｇＧ亚类同种异型覆盖面。
Human serum immunoglobulin G (IgG) subclass concentrations were determined by ELISA using monoclonal antibodies (mAb) and WHO67 / 97 standard sera. The results showed that the method was sensitive (the lowest measurable value was 2.1ng / ml, the measurable range was 100 times), and the accuracy was accurate (the average coefficient of variation was ≤8.06% in different batches). The McAb specificity and reactivity were good, and the sum of IgG subclasses was closely related to the total IgG value measured by routine single-spike method (r = 0.96, P <0.01). Emphasized that the monoclonal antibody coated on BSA-glutaraldehyde-treated microplates, can significantly improve the mAb adsorption and reactivity; two monoclonal antibody hybrid coating can increase the IgG subclass allotype coverage.