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目的研究中国苏皖地区汉族人群P53 codon 72多态性与前列腺癌(PCa)易感性的关系。方法采用病例-对照研究,提取209例PCa患者(病例组)和23 5例非肿瘤患者(对照组)外周血基因组DNA,应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析两组P53 codon 72位点的多态性,比较不同基因型与PCa易感性的关系。结果以携带Pro/Pro基因型的对象为参照组,携带Arg/Pro基因型者患PCa的风险提高89%(OR=1.89,95%CI:1.20～2.97);携带Arg/Arg基因型者患PCa的风险提高101%(OR=2.01,95%CI:1.11～3.64)。而携带Arg等位基因者(Arg/Pro+Arg/Arg)患PCa的风险是携带Pro/Pro基因型的1.94倍(OR=1.94,95%CI:1.25～2.99)。在高龄(>70岁)、高体重指数(>23kg/m~2)、非吸烟、非饮酒和无肿瘤家族史5组人群中,携带Arg等位基因的个体发病风险是Pro/Pro基因型个体的2倍以上,调整后的OR(95%CI)分别为2.22(1.24～3.96)、2.24(1.30～3.8 8)、2.60(1.27～5.31)、2.23(1.19～4.18)、2.18(1.33～3.57)。结论 P53 codon 72与苏皖地区汉族人群PCa的易感性有关,Arg/Pro、Arg/Arg可能是PCa的易感基因型。
Objective To investigate the relationship between P53 codon 72 polymorphism and the susceptibility to prostate cancer (PCa) in Chinese Han population in Jiangsu and Anhui Provinces. Methods A total of 209 cases of PCa (case group) and 23 5 cases of non-tumor (control group) peripheral blood genomic DNA were extracted by case-control study. PCR-RFLP ) Analysis of the two groups of P53 codon 72 polymorphism, compared with different genotypes and PCa susceptibility. Results The risk of PCa in patients with Arg / Pro genotype increased by 89% (OR = 1.89, 95% CI: 1.20-2.97) with reference to the group carrying Pro / Pro genotype. Patients with Arg / Arg genotype The risk of PCa increased by 101% (OR = 2.01, 95% CI: 1.11 to 3.64). However, the risk of PCa in patients with Arg allele (Arg / Pro + Arg / Arg) was 1.94 times higher than those carrying Pro / Pro genotype (OR = 1.94, 95% CI: 1.25-2.99). Among the five age groups (> 70 years old), high body mass index (> 23 kg / m ~ 2), non-smoking, non-alcohol consumption and no family history of cancer, the risk of developing Arg allele was Pro / Pro genotype (95% CI) were 2.22 (1.24-3.96), 2.24 (1.30-3.88), 2.60 (1.27-5.31), 2.23 (1.19-4.18), 2.18 3.57). Conclusion P53 codon 72 is associated with susceptibility to PCa in Han population in Jiangsu and Anhui Provinces. Arg / Pro and Arg / Arg may be susceptible to PCa.