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致病疫霉Phytophthora infestans属于异宗配合卵菌,当A1、A2两种交配型同时存在时,可以进行有性生殖,产生卵孢子。检测疫霉菌交配型的传统方法是采用对峙培养,这种方法耗时长并且需要标准的A1、A2交配型菌株作为参照。因此,人们希望开发出更加简便和快捷的可直接基于核苷酸序列差异的分子检测方法。目前,已报道了3个与致病疫霉交配型紧密连锁的分子标记可用于交配型的检测。本研究用64株致病疫霉菌比较了3种基于交配型分子标记的检测方法与传统方法检测的结果。结果显示,依据分子标记的3种分子检测方法与传统对峙培养方法测定的交配型结果一致率为61%–73%,而且3种分子检测方法都不能检测出自育菌株。因此,致病疫霉交配型的分子检测方法还有待进一步研究。
Phytophthora infestans belongs to the heterozygote with oomycetes. When both mating types of A1 and A2 are present, sexual reproduction can occur and produce oospores. The traditional method for detecting the mating type of Phytophthora infestans is confrontation culture, which takes a long time and requires standard A1, A2 mating strains as a reference. Therefore, one hopes to develop a simpler and faster molecular detection method based on nucleotide sequence differences directly. Currently, three molecular markers closely linked to P. infestans mating type have been reported for mating type detection. In this study, 64 strains of Phytophthora infestans were used to compare the results of three detection methods based on mating type molecular markers and traditional methods. The results showed that the concordant rate of the three mating methods based on molecular markers was 61% -73% compared with that of the traditional confrontation culture method. Moreover, all the three molecular methods could not detect the self-bred strains. Therefore, the mating type of molecular detection of Phytophthora infestans needs further study.