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目的了解长角血蜱的遗传多样性和不同地理种群间的系统发育关系,为长角血蜱及蜱媒病的防控奠定基础。方法基于45条长角血蜱COI序列,采用PAUP*4.0构建最大似然树,用DnaSP 5.0计算单倍型多样性和核苷酸多样性。结果长角血蜱单倍型多样性Hd为0.810~1.000,核苷酸多样性π为0.218-1.990,Fst值为0.0213-0.4522,Tajima’s D为-0.7245,Fu’s Fs为-2.454,分析结果均表明中国长角血蜱不同地理种群间无明显分化。结论系统发育分析和单倍型网络图表明长角血蜱不同地理种群正在发生扩张,但尚无明显分化。
Objective To understand the genetic diversity of H. longicornis and its phylogenetic relationship among different geographical populations, and lay a foundation for the prevention and control of P. longicornis and tick-borne disease. Methods Based on the COI sequences of 45 H. longicornis, the maximum likelihood tree was constructed using PAUP * 4.0, and the haplotype diversity and nucleotide diversity were calculated using DnaSP 5.0. Results The haplotype diversity of H. longicornis was 0.810-1.000, the nucleotide diversity π was 0.218-1.990, the Fst value was 0.0213-0.4522, Tajima’s D was -0.7245, and Fu’s Fs was -2.454. The results of the analysis showed that There was no obvious differentiation among different geographical populations of H. longicornis. Conclusions Phylogenetic analysis and haplogroup network showed that different geographical populations of H. oriensis were expanding, but there was no obvious differentiation.