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目的探讨p38丝裂原活化蛋白激酶(p38 MAPK)在慢性非细菌性前列腺炎(CNP)大鼠模型中的表达,与细胞因子肿瘤坏死因子-α(TNF-α)、环氧合酶-2(COX-2)、基质金属蛋白酶-9 (MMP-9)的关系及它们在发病机制中可能的作用。方法将成年雄性SD大鼠去势后皮下注射苯甲酸雌二醇连续4周建模,对照组不做任何处理。HE染色,光镜下观察两组前列腺组织的病理学表现,免疫组化检测两组TNF-α、MMP-9、COX-2、磷酸化p38(p-p38)蛋白的表达,逆转录-聚合酶链反应(RT-PCR)检测p38 mRNA的水平变化。结果光镜下对照组前列腺组织无炎症反应,模型组表现为明显的炎症反应。对照组前列腺组织TNF-α、MMP-9、COX-2、p-p38蛋白表达量极少,而模型组明显增加,两组差异有统计学意义(P<0.05)。对照组p38 mRNA的表达为模型组的37.3%。结论在大鼠CNP中p38 MAPK信号转导途径被激活。细胞因子TNF-α、MMP-9、COX-2的表达可能是通过p38 MAPK调节。这为CNP的研究和治疗提供了一条新的途径。
Objective To investigate the expression of p38 mitogen-activated protein kinase (p38 MAPK) in chronic nonbacterial prostatitis (CNP) rat model, and to investigate the relationship between p38 MAPK and cytokines such as tumor necrosis factor-α (TNF- (COX-2), matrix metalloproteinase-9 (MMP-9) and their possible role in the pathogenesis. Methods Adult male Sprague-Dawley rats were subcutaneously injected with estradiol benzoate for 4 weeks after castration, without any treatment in the control group. HE staining and pathological examination of the two groups of prostate tissue were performed under light microscope. The expressions of TNF-α, MMP-9, COX-2 and p38 protein in the two groups were detected by immunohistochemistry, Enzyme-linked reaction (RT-PCR) detection of p38 mRNA level changes. Results There was no inflammatory reaction in the control group prostate tissue under light microscope. The model group showed obvious inflammatory reaction. The expression of TNF-α, MMP-9, COX-2 and p-p38 protein in prostate tissue of the control group was extremely low, while the model group was significantly increased. There was significant difference between the two groups (P <0.05). The expression of p38 mRNA in the control group was 37.3% of the model group. Conclusion The p38 MAPK signal transduction pathway is activated in rat CNP. The expression of cytokines TNF-α, MMP-9 and COX-2 may be regulated by p38 MAPK. This provides a new way for CNP research and treatment.