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我们使用一步法扩增得到乙型肝炎病毒(HBV)基因组全序列,与T载体连接,并转化入自制的超高效TOPO10感受态细胞,获得了较高的转化效率。为今后深入进行HBV全基因组的研究提供了基础和工具。
We amplified the complete genome of Hepatitis B virus (HBV) using one-step amplification and ligated it with the T vector and transformed it into a homemade super-efficient TOPO10 competent cell with high transformation efficiency. It will provide the foundation and tools for further research on HBV whole genome in the future.