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本研究利用ＤＤ－ＰＣＲ技术分析了光敏核不育籼稻ＨＳ－１可育和不育幼穗ｍＲＮＡ的表达差异。结果表明：在１４８个ＤＤ－ＰＣＲ反应中，１０个反应可扩增出有差异且可重复的ｃＤＮＡ片段，其中４个反应可扩增出１～４条差异片段，６个反应可扩增出４个以上的差异片段，且这些差异片段可只出现在可育幼穗中或同时出现在可育和不育幼穗中。共获得４３个差异ｃＤＮＡ片段，其中２１个可能与光敏核不育性相关。本研究认为，寻找光敏核不育基因在可育和不育条件下差异表达的起始发育时期是从ｍＲＮＡ水平分离这一基因的关键。光敏核不育基因的表达可能会引起其它一些ｍＲＮＡ的合成，从而产生较多的差异片段，进一步鉴定这些差异片段与不育的关系十分重要。
In this study, DD-PCR was used to analyze the difference of mRNA expression in fertile and sterile young plants of CMS line HS-1. The results showed that among the 148 DD-PCR reactions, 10 reactions could amplify the differentially and reproducible cDNA fragments, among which 4 fragments amplified 1 ~ 4 fragments and 6 fragments amplified More than 4 different fragments, and these differential fragments may appear only in fertile panicles or in both fertile and sterile panicles. A total of 43 differentially expressed cDNA fragments were obtained, of which 21 were probably related to photo-sensitive genic male sterility. In this study, we found that finding the initial developmental stage of differential expression of the photogene-sensitive genic male sterile gene under fertile and sterile conditions is the key point to isolate this gene from mRNA level. The expression of photogene-sensitive genic male sterility genes may cause the synthesis of some other mRNAs, resulting in more difference fragments. It is very important to further identify the relationship between these difference fragments and infertility.