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建立淫羊藿及其制剂中淫羊藿苷的毛细管胶束电动色谱分析方法。电泳缓冲液由2 0mmol/L四硼酸钠、4 5mmol/L脱氧胆酸钠、φ =35%乙腈 (pH =8.5)组成 ,以卡马西平为内标 ,工作电压 2 5kV ,检测波长 2 70nm。该方法在 5～ 80mg/L范围内呈线性 ,r =0 .9998,日内、日间RSD1.9%～ 4 .8%。毛细管胶束电动色谱法专一性强 ,精密度高 ,是一种较好的中药有效成分检测方法。
Establishment of Capillary Micellar Electrokinetic Chromatography of Icariin in Epimedium and Its Preparations. The electrophoresis buffer consisted of 20 mmol / L sodium tetraborate, 45 mmol / L sodium deoxycholate and φ = 35% acetonitrile (pH = 8.5). Carbamazepine was used as an internal standard and the working voltage was 25 kV and the detection wavelength was 2 70 nm . The method in the range of 5 ~ 80mg / L linear, r = 0.9998, intraday, daytime RSD 1.9% ~ 4.8%. Capillary micellar electrokinetic chromatography has the advantages of strong specificity and high precision, and is a good method for detecting the active ingredients of traditional Chinese medicine.