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用MTT法检测中药AF-7,丝裂霉素(MMC),5-氟脲嘧啶(5-Fu)在单纯用药及加温与加药共同应用,对人结肠高分化腺癌细胞系THC8908的作用,并用抗多药耐药(MDR)基因表达蛋白P170单抗及免疫组化法,检测该细胞在上述条件下(MDR)基因表达的异同。结果发现:1)加温可增强AF-7、MMC和5-Fu对肿瘤细胞的杀伤作用。2)免疫组化染色揭示,正常培养条件下该细胞MDR为中度表达,经加142.5℃1小时再培养72小时其MDR则不表达。单独用药各组均为中度表达,而加温与加药共同应用组均为低度表达,且MMC组MDR表达的阳性细胞数高于AF-7组。研究证明加温能明显抑制MDR基因表达,从而提高化疗效果。
MTT assay was used to detect the traditional Chinese medicine AF-7, mitomycin (MMC), and 5-fluorouracil (5-Fu) in combination with the use of drugs and heating and dosing, and it was applied to the human colonic well-differentiated adenocarcinoma cell line THC8908. The effects of the MDR gene expression and the immunohistochemical method on the expression of the MDR gene were compared. The results showed that: 1) Heating can enhance the killing effect of AF-7, MMC and 5-Fu on tumor cells. 2) Immunohistochemical staining revealed that the MDR was moderately expressed under normal culture conditions, and MDR was not expressed after 142.5°C for 1 hour and then 72 hours of culture. All the groups were moderately expressed, while the combined groups of warming and adding drugs were all low expression, and the number of MDR positive cells in MMC group was higher than AF-7 group. Studies have shown that heating can significantly inhibit the expression of MDR genes, thereby increasing the effect of chemotherapy.