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目的:了解多发性骨髓瘤(MM)患者外周血中黏膜相关组织淋巴瘤异位基因1(MALT1)、A20与核转录因子κB(NF-κB)基因的表达特点。方法:采用SYBR GreenⅠ荧光实时定量PCR和相对定量分析法检测20例MM患者(Ⅰ期7例,Ⅱ期3例,Ⅲ期10例)及21例健康人外周血单个核细胞(PBMC)中MALT1、A20和NF-κB基因的表达情况。以β2微球蛋白基因(β2m)作为内参;采用相对定量公式:α~(-△Ct)×100%,分别计算MALT1、A20和NF-κB基因的表达水平。结果:MM患者PBMC中的MALT1和A20基因的表达水平较健康人都有明显的降低(P=0.000),而MM中NF-κB基因的表达水平较正常人有所升高,但无统计学差异(P>0.05)。在健康人组,MALT1和NF-κB的表达水平呈现正相关(P=0.001,r=0.666),而在MM组,MALT1、A20与NF-κB 3种基因均不存在明显相关性。同时,Ⅰ、Ⅱ、Ⅲ期MM患者MALT1基因的表达量较健康人明显降低(P<0.05),A20基因的表达量较健康人明显降低(P<0.01),而Ⅰ、Ⅱ、Ⅲ期MM患者MALT1、A20和NF-κB之间的表达情况均无统计学差异。结论:当MALT1-A20介导的细胞免疫和炎症的信号通路发生异常改变时,可能与MM的发生发展有着密切关系。
Objective: To investigate the expression of MALT1, A20 and NF-κB in peripheral blood of patients with multiple myeloma (MM). Methods: SYBR Green Ⅰ real-time quantitative PCR and relative quantitative assay were used to detect the expression of MALT1 in peripheral blood mononuclear cells (PBMCs) of 20 patients with MM (7 in stage Ⅰ, 3 in stage Ⅱ, 10 in stage Ⅲ) and 21 in healthy subjects , A20 and NF-κB gene expression. Β2 microglobulin gene (β2m) was used as an internal reference. The relative quantification formula: α ~ (- △ Ct) × 100% was used to calculate the expression of MALT1, A20 and NF-κB. Results: The expression of MALT1 and A20 genes in PBMC of MM patients was significantly lower than that of healthy people (P = 0.000), while the expression of NF-κB gene in MM patients was higher than that of normal controls Difference (P> 0.05). There was a positive correlation between the expression levels of MALT1 and NF-κB in healthy people (P = 0.001, r = 0.666). However, there was no significant correlation between MALT1, A20 and NF-κB in MM patients. Meanwhile, the expression of MALT1 gene in patients with stage I, II, and III MM was significantly lower than that in healthy people (P <0.05), and the expression of A20 gene was significantly lower than that in healthy people (P <0.01) There was no statistical difference in the expression of MALT1, A20 and NF-κB in patients. Conclusion: MALT1-A20 may play an important role in the pathogenesis of MM when abnormal signal transduction pathway is involved in cellular immunity and inflammation.