为了解Cu,Zn-SOD酶中Cu周围环境对催化O_2~-歧化作用活性的影响,本文用NBT法测定了Cu,Zn-SOD酶及一系列咪唑桥异核模型配合物催化O_2~-歧化作用的活性,并用ESR方法鉴测了其中一个配合物与O_2~-作用的中间过程。结果表明,具有平面四方构型或轴向有弱配位H_2O或ClO_4~-基团的四方锥构型的Cu~(2+)配合物具有较高活性,说明配合物催化歧化O_2~-过程中,Cu~(2+)可能首先以轴向与O_2~-配位,形成Cu~(2+)-O_2~-中间化合物,紧接着Cu~(2+)被还原成Cu~+。 In order to understand the effect of Cu environment on the activity of Cu 2+ and Zn-SOD in catalyzing O_2-disproportionation activity, we used the NBT method to determine the catalytic activity of Cu, Zn-SOD and a series of imidazole bridges The activity of one of the complexes with O_2 ~ - was evaluated by ESR method. The results show that the tetragonal conformation Cu 2+ complexes with planar tetragonal structure or axial weakly coordinated H_2O or ClO_4 ~ - groups have high activity, indicating that the complexes catalyze the disproportionation of O_2 ~ -process , Cu ~ (2+) may first coordinate with O_2 ~ - axially to form Cu ~ (2 +) - O_2 ~ - intermediate compound, followed by Cu ~ (2+) reduction to Cu ~ +.