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目的利用甘蔗镰孢Fusarium sacchari对人参茎叶皂苷进行生物转化。方法转化产物通过硅胶柱色谱进行分离,经理化常数和光谱分析鉴定化合物的结构。结果从人参茎叶皂苷的Fusarium sacchari转化产物中分离鉴定了10个化合物,分别为20(S)-人参二醇(1)、20(S)-原人参二醇(2)、20(R)-原人参二醇(3)、20(S)-人参三醇(4)、20(S)-原人参三醇(5)、20(R)-原人参三醇(6)、20(S)-原人参二醇-20-O-β-D-吡喃葡萄糖苷(CK)(7)、人参皂苷F1(8)、人参皂苷Rh1(9)和人参皂苷Rg1(10)。结论化合物1~10为首次从人参茎叶皂苷的Fusarium sacchari生物转化产物中分离得到,且Fusarium sacchari可转化人参茎叶皂苷生成稀有抗肿瘤皂苷,是一种具有开发价值的稀有活性菌株。
Objective To transform the ginsenosides with Fusarium sacchari. Methods The products were separated by column chromatography on silica gel. The structures of the compounds were identified by physicochemical constants and spectral analysis. Results Ten compounds were isolated from the Fusarium sacchari transformation products of ginseng stem and leaf saponin and were identified as 20 (S) - ginseng diol (1), 20 (S) - protopanaxadiol (2), 20 - Protopanaxadiol (3), 20 (S) - Panaxtriol (4), 20 (S) - Protopanaxol (5), 20 (R) ) - Protopanaxel-20-O-β-D-glucopyranoside (CK) (7), ginsenoside F1 (8), ginsenoside Rh1 (9) and ginsenoside Rg1 (10). Conclusions Compounds 1-10 were isolated from the Fusarium sacchari bioconversion product of ginseng stem and leaf saponin for a first time. Fusarium sacchari transformed ginseng stem and leaf saponin to produce rare anti-tumor saponin, which is a rare and active strain with development value.