论文部分内容阅读
目的探讨糖尿病骨骼肌碱性成纤维细胞生长因子(bFGF)及血管内皮生长因子(VEGF)表达的变化及其在糖尿病骨骼肌病变中的作用。方法选用Wistar大鼠,随机分成4组:正常对照组、单纯后肢缺血组、糖尿病组和糖尿病后肢缺血组。以四氧嘧啶50mg/kg尾静脉注射制造糖尿病模型。糖尿病造模后2周结扎右股动脉制造缺血模型。10周后,观察腓肠肌病理形态学改变,同时采用免疫组化方法检测bFGF和VEGF蛋白表达情况。结果糖尿病组骨骼肌表现为普遍性肌萎缩,肌纤维变性坏死。单纯缺血组和糖尿病组bFGF蛋白表达减弱(表达阳性率分别为53.66%和35.51%),糖尿病后肢缺血组bFGF的阳性率为25.42%,下降明显穴P<0.01雪。糖尿病缺血组VEGF表达阳性率为20.68%,低于单纯缺血组(54.04%,P<0.01)。结论高血糖和缺血引起肌肉中bFGF及VEGF等生长因子的变化可能是糖尿病骨骼肌萎缩的重要原因之一。
Objective To investigate the changes of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in diabetic skeletal muscle and its role in the pathogenesis of diabetic skeletal muscle. Methods Wistar rats were randomly divided into 4 groups: normal control group, hind limb ischemia group, diabetic group and diabetic hindlimb ischemia group. Alloxan 50mg / kg tail vein injection to create diabetes model. Ligation of the right femoral artery 2 weeks after modeling of diabetes created a model of ischemia. Ten weeks later, the pathological changes of gastrocnemius muscle were observed. Meanwhile, the expression of bFGF and VEGF protein was detected by immunohistochemistry. Results The skeletal muscle in diabetic group showed generalized muscle atrophy and myofibrosis. The expression of bFGF protein was decreased (53.66% and 35.51% respectively) in ischemia group and diabetic group. The positive rate of bFGF in diabetic hindlimb ischemia group was 25.42%, significantly decreased (P <0.01). The positive rate of VEGF expression in diabetic ischemic group was 20.68%, lower than that in simple ischemic group (54.04%, P <0.01). Conclusion The changes of bFGF, VEGF and other growth factors in muscle caused by hyperglycemia and ischemia may be one of the important causes of diabetic skeletal muscle atrophy.