松材线虫病是松树的一种毁灭性病害。本文对接种松材线虫后3~4年生黑松茎部的一些生理生化指标的变化进行了研究。结果显示,接种线虫后,松苗的可溶性糖含量降低,在病情发展中期和后期比早期降低更显著。松苗的可溶性蛋白质和抗坏血酸含量逐渐降低,游离氨基酸含量也降低。松树茎部的可溶性蛋白质含量可用于病害的早期诊断。此外,对这4个生理生化指标在病害致病机理中的作用进行了讨论。 Pine wilt disease is a devastating disease of pine trees. In this paper, the changes of some physiological and biochemical indexes of 3 ~ 4-year-old black pine stems after inoculated with pine wood nematode were studied. The results showed that after nematode inoculation, the content of soluble sugar of pine seedlings decreased, which was more significant in the mid and late stages of disease than earlier. The content of soluble protein and ascorbic acid in pine seedlings gradually decreased, and the content of free amino acids also decreased. The soluble protein content of the stem of pine can be used for the early diagnosis of the disease. In addition, the roles of these four physiological and biochemical indexes in the pathogenic mechanism of disease were discussed.