论文部分内容阅读
目的:观察GDNF基因修饰的骨髓间充质干细胞(BMSCs)对脑出血大鼠脑源性神经营养因子(BDNF)及其受体的影响。方法:通过脑立体定位仪向SD大鼠脑尾壳核注射胶原酶和肝素建立脑出血动物模型,并随机分为BMSCs组、GDNF/BMSCs组和生理盐水组共3组,并于建模后第3d在脑出血部位分别移植BMSCs、GDNF/BMSCs以及生理盐水;各组又根据细胞移植后再喂养时间的不同(1周、2周)分为2个亚组,每亚组8只大鼠。采用RT-PCR法观察BDNF和受体TrkB mRNA的表达;免疫组织化学染色观察BDNF蛋白的表达。结果:与生理盐水组和BMSCs组相比,BDNF及受体TrkB mRNA的表达上调。在1周和2周时间点,GDNF/BMSCs组与BMSCs组和生理盐水组相比BDNF阳性细胞数明显增加。结论:GDNF基因修饰的BMSCs移植治疗脑出血大鼠能增强BDNF及其受体的表达,有利于大鼠神经功能恢复。
Objective: To observe the effect of GDNF gene modified bone marrow mesenchymal stem cells (BMSCs) on brain-derived neurotrophic factor (BDNF) and its receptor in intracerebral hemorrhage rats. Methods: Intracerebral hemorrhage was induced by injection of collagenase and heparin into the caudate putamen of SD rats by brain stereotaxic instrument. The rats were randomly divided into 3 groups: BMSCs group, GDNF / BMSCs group and saline group. After modeling On the 3rd day, BMSCs, GDNF / BMSCs and saline were respectively transplanted into the intracerebral hemorrhage sites. Each group was divided into 2 subgroups according to the different feeding time (1 week and 2 weeks) . The expression of BDNF and TrkB mRNA was detected by RT-PCR. The expression of BDNF protein was observed by immunohistochemical staining. Results: Compared with saline group and BMSCs group, BDNF and TrkB mRNA expression were up-regulated. At 1 week and 2 weeks, the number of BDNF positive cells in GDNF / BMSCs group was significantly higher than that in BMSCs group and saline group. CONCLUSION: GDNF gene-modified BMSCs transplantation can enhance the expression of BDNF and its receptor in rats with intracerebral hemorrhage and is beneficial to the recovery of neurological function in rats.