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采用活化的高效液相膜亲和介质为固相化载体，与纯化的人ＩｇＭ 抗体进行交联，制备了ＩｇＭ 高效膜亲和色谱柱，与ＩｇＧ膜亲和柱比较了交联效率，并用相应的抗抗体通过ＨＰＬＣ测定了两者的亲和能力。在相同条件下，ＩｇＭ 的交联效率为ＩｇＧ的一半，但最大亲和容量却与之相近，并且在一定范围内可以完全分离血清中抗ＩｇＭ 抗体。结果表明：ＩｇＭ 高效膜色谱柱可以高效快速亲和分离相应抗体
An activated high-performance liquid phase membrane affinity medium is used as a solid phase carrier and is cross-linked with the purified human IgM antibody to prepare an IgM high-performance membrane affinity chromatography column. The efficiency of crosslinking is compared with that of an IgG membrane affinity column, The anti-antibodies were measured by HPLC affinity between the two. Under the same conditions, IgM cross-linking efficiency of IgG half, but the maximum affinity capacity is similar, and within a certain range of serum anti-IgM antibodies can be completely separated. The results showed that the IgM high efficiency membrane column can efficiently and quickly separate the corresponding antibodies