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目的检测载脂蛋白M(ApoM)基因rs707921位点的基因多态性,探讨其与冠心病(CHD)的相关性。方法采用单荧光标记探针技术检测111例CHD患者及248例对照组的ApoM rs707921位点单核苷酸多态性,分析其基因型和等位基因频率的分布情况。结果 ApoM rs707921位点3种基因型(AA型、AC型和CC型)在CHD组中分布频率为1.8%、13.5%和84.7%,在对照组中分布频率为2.0%、25.4%和72.6%,两组差异有统计学意义(P=0.039);ApoM rs707921位点A、C等位基因的频率在CHD组和对照组中分布频率分别为8.6%、91.4%和14.7%、85.3%,两组比较差异有统计学意义(P=0.023)。CHD组中AC+AA基因型者甘油三酯水平显著低于CC基因型者(P=0.043)。CHD组rs707921位点多态性不同基因型间冠状动脉病变严重程度差异无统计学意义(P>0.05)。结论ApoM基因rs707921位点A等位基因可能降低CHD的发病风险,但与CHD的严重程度无关。
Objective To detect the gene polymorphism of rs707921 in apolipoprotein M (ApoM) gene and to explore its relationship with coronary heart disease (CHD). Methods ApoM rs707921 single nucleotide polymorphism was detected in 111 CHD patients and 248 control subjects by single fluorescent probe technique. The distribution of genotype and allele frequency was analyzed. Results The frequencies of ApoM rs707921 locus were 1.8%, 13.5% and 84.7% in the CHD group and 2.0%, 25.4% and 72.6% in the control group, respectively. (P = 0.039). The frequencies of A and C alleles in ApoM rs707921 were 8.6%, 91.4% and 14.7%, 85.3% respectively in CHD group and control group The difference was statistically significant (P = 0.023). In the CHD group, triglyceride levels were significantly lower in AC + AA genotypes than in CC genotypes (P = 0.043). There was no significant difference in the severity of coronary artery lesions between rs707921 polymorphism genotypes in CHD group (P> 0.05). Conclusion The A allele of rs707921 ApoM gene may reduce the risk of CHD, but not with the severity of CHD.