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目的探讨α-细辛醚对Fmr1基因敲除小鼠的自主活动的干预作用。方法选取30日龄Fmr1基因敲除小鼠(KO小鼠)和FVB野生型小鼠(WT小鼠)为研究对象,将KO和WT两种类型的小鼠分别分为7小组,每组15只。其中1组作为对照组给予生理盐水,另外6小组连续腹腔注射不同剂量α-细辛醚(3 mg/kg、6 mg/kg、9 mg/kg、12 mg/kg、24 mg/kg、36mg/kg)5天,用药第5天进行自主活动行为学实验,观察α-细辛醚能否改善KO鼠的过度活动的表型。结果在行为学自主活动实验中,KO鼠的活动次数比WT鼠的活动次数多,站立次数比WT鼠的站立次数少,差异具有统计学意义(P<0.05);使用α-细辛醚后,KO鼠的活动次数明显减少,站立次数明显增多,差异均具有统计学意义(P均<0.05)。结论α-细辛醚能改善KO鼠的的活动过度的表型,可能对Fmr1基因敲除小鼠有治疗作用。
Objective To investigate the effects of α-asarone on the autonomic activity of Fmr1 knockout mice. Methods 30-day-old Fmr1 knockout mice (KO mice) and FVB wild-type mice (WT mice) were selected as experimental subjects. The KO and WT mice were divided into 7 groups of 15 only. One group was given normal saline as the control group, the other 6 groups were injected intraperitoneally with different dosages of α-asarone (3 mg / kg, 6 mg / kg, 9 mg / kg, 12 mg / kg, 24 mg / / kg) for 5 days. The 5th day of drug administration was used to conduct autonomous behavioral experiments to observe whether α-asarone could improve the over-activity phenotype in KO rats. Results In the experiment of behavioral autonomy, KO rats had more activities than WT rats, and their standing times were less than that of WT mice (P <0.05). After using α-asarone , KO mice significantly reduced the number of activities, standing significantly increased, the differences were statistically significant (P all <0.05). Conclusion α-asarone can improve the over-activity phenotype of KO mice and may have a therapeutic effect on Fmr1 knock-out mice.