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The relative molecular mass of Arbutin is small.Both fluorolabeling and radiolabeling may affect its properties and functions.Therefore, the immunoassay of Arbutin was studied.Arbutin was coupled to bovine serum albumin to get the Arbutin-BSA conjugate with high molar ratio of Arbutin to BSA.Two rabbits were injected with the conjugate to develop the anti-Arbutin serum.Ammonium sulfate precipitation and affinity chromatography were used to purify the antibody.Double agar diffusion test and enzyme-linked immunosorbent assay (ELISA) were adopted to identify the antibody titer.The results demonstrated that the purity and activity of the antibody are high.The method proposed is satisfactory for the immunological detection of Arbutin.