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采用人胚大脑神经细胞体外无血清原代分离培养方法,对牛磺酸在人脑神经细胞分化成熟过程中的作用进行了研究。结果发现,牛磺酸对神经细胞14-3-2蛋白的合成具有明显的促进作用,表现为实验组与对照组神经细胞14-3-2蛋白的出现分别于细胞培养后的第15天和第20天。而且实验组神经细胞的14-3-2蛋白的含量呈直线增长趋势。提示,牛磺酸在人脑神经细胞的分化成熟过程中发挥着重要的作用。
The primary culture of human embryonic brain neurons in vitro without serum was used to study the role of taurine in the differentiation and maturation of human brain neurons. The results showed that taurine on neuronal 14-3-2 protein synthesis has a significant role in promoting the performance of the experimental group and the control group of neuronal 14-3-2 protein appeared in cell culture after 15 days and Day 20 Moreover, the content of 14-3-2 protein in the experimental group neurons increased linearly. Tip, taurine in human brain cells play an important role in differentiation and maturation.