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在谷类作物的遗传研究和育种过程中,由于育种初期选择单株或株系数量较多,在进行品质育种时需要快速准确地测定蛋白质等的方法。本研究是结合一个遗传试验,利用GXDL—202型蛋白质赖氨酸分析仪测定小麦亲本P_1(早5)与P_2(蛐选64)杂交的F_1、F_2及回交的B_1和B_2六个世代共220株的粗蛋白质含量。将每株一定量籽粒粉碎后,取两个样品用GXDL—202测定反应液光密度,得一平均(二次)光密度,同时在该株粉碎籽粒中再取二个样品用凯氏法(中华人民共和国国家标准1982)测得粗蛋白质含量得一平均(二次)值。分别对小麦各世代光密度(x)与蛋白质含量(y)的线性回归和相关分析,结果如表1。
In the process of genetic research and breeding of cereals, there are a large number of individual plants or lines at the early breeding stage, and the method of quickly and accurately measuring proteins and the like during quality breeding is required. In this study, a genetic experiment was carried out to determine the F_1, F_2 and the B_1 and B_2 backcrossing lines of P 1 (early 5) and P_2 (P64) by GXDL-202 protein lysine analyzer 220 crude protein content. After crushing a certain amount of each seed grain, two samples were taken to measure the optical density of the reaction solution by GXDL-202 to obtain an average (secondary) optical density, and two more samples were taken from the crushed grain of the strain. Kjeldahl People’s Republic of China National Standard 1982) measured crude protein content had an average (secondary) value. Linear regression and correlation analysis of optical density (x) and protein content (y) of each generation of wheat were carried out. The results are shown in Table 1.