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目的建立高效液相色谱荧光法测定游泳池水中尿素的方法。方法水样中的尿素,在酸性条件下用呫吨氢醇衍生,衍生物经液相色谱柱分离,以乙腈和醋酸钠为流动相,梯度洗脱,荧光检测器检测波长EX=213 nm和EM=308 nm。结果水中尿素的浓度在范围内,峰面积与浓度呈良好的线性关系,r2=0.999 8,方法的检出限为0.48 mg/kg,添加高、中、低3个浓度时,回收率在84.0%~102.8%之间。结论实验表明,该方法具有方法简单、可靠、准确,可适用于游泳池水中尿素含量的批量检测。
Objective To establish a HPLC method for the determination of urea in swimming pool water. Methods Urea in water samples was derivatized with xanthene by acidic conditions. The derivatives were separated by liquid chromatography with acetonitrile and sodium acetate as mobile phases and gradient elution. The detection wavelength of fluorescence detector was EX = 213 nm and EM = 308 nm. Results The concentration of urea in water showed a good linear relationship between the peak area and concentration, r2 = 0.999 8 and the detection limit of the method was 0.48 mg / kg. The recoveries were 84.0 at the three concentrations of high, medium and low % ~ 102.8% between. Conclusion The experiment shows that this method is simple, reliable and accurate and can be applied to batch detection of urea in swimming pool water.