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研究原生于甘洛县的黑苦荞种籽在原生地和非原生地种植后,分别采收开花前的茎叶,测定茎叶总黄酮、膳食纤维的含量,再比较60%乙醇提取物的功效成分和降血糖功能,探讨非原生地茎叶提取物的降血糖功能及其代替原生地资源的可行性。腹腔注射四氧嘧啶(180 mg/kg)建立小鼠糖尿病模型,将两种来源的提取物(1000 mg生药/kg、500 mg生药/kg)分别灌胃,阳性对照组给予中成药的糖尿乐(300 mg/kg),连续12 d灌胃,测定处理前后的空腹血糖值,每4 d称重一次。结果表明,灌胃两种来源的黑苦荞茎叶提取物均能明显抑制高血糖小鼠体重的降低,对其高血糖有显著的降低作用,并可增强其葡萄糖耐受量。两种来源黑苦荞茎叶的膳食纤维含量差异显著,总黄酮含量差异也显著,其中,除芦丁的差异显著外,槲皮素和D-手性肌醇的差异不显著。两种来源黑苦荞茎叶经乙醇提取后,提取物的总黄酮和D-手性肌醇,芦丁、槲皮素的差异均不显著,动物试验表明两种提取物都有降血糖功能,所以,非原生地黑苦荞茎叶可代替原生地的,用于开发降血糖的功能产品。
The study was to investigate the effects of 60% ethanol extract on the content of total flavonoids and dietary fiber in stems and leaves of black buckwheat seeds native to Gaoluo County after they were planted in native and non-native plants. Composition and hypoglycemic function to explore the non-native stem and leaf extract hypoglycemic function and its feasibility of replacing native resources. The model of diabetic mice was established by intraperitoneal injection of alloxan (180 mg / kg). The two kinds of extracts (1000 mg crude drug / kg, 500 mg crude drug / kg) were intragastrically administered respectively. The positive control group was given Chinese patent medicine (300 mg / kg) for 12 days. The fasting blood glucose before and after treatment was measured and weighed every 4 days. The results showed that both gavage stem and leaf extracts from both sources could significantly reduce the body weight of hyperglycemic mice, significantly reduce the hyperglycemia and enhance the glucose tolerance. The content of dietary fiber of black tartary buckwheat stem and leaf differed significantly among the two sources, and the content of total flavonoids was also significant. The difference of quercetin and D-chiro-inositol was not significant except for the significant difference of rutin. There was no significant difference in total flavonoids and D-chiro-inositol, rutin, quercetin between the two sources after ethanol extraction from the stems and leaves of black tartary buckwheat. Animal experiments showed that both extracts had hypoglycemic function , So non-native black bitter buckwheat stems and leaves instead of native, for the development of hypoglycemic functional products.