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目的研究慢性肝病和原发性肝癌病人血清、组织中纳米细菌(NB)感染,为其发生机制提供新的认识。方法 55例慢性肝病(25例慢性乙肝和30例肝炎后肝硬化)、43例肝癌病人和336例健康人血清采用 ELISA、免疫组化和钙染色;53例慢性肝病(28例慢性乙肝和25例肝硬化)、43例肝癌和15例对照肝组织行免疫组化染色,部分阳性组织透射电镜观察。结果(1)慢性肝病、肝癌病人和健康人血清 ELISA 检测阳性率分别为20.0%、9.3%和8.0%,慢性肝病 NB 感染率高于健康人(P<0.05)。免疫组化染色感染率分别为14.5%、4.7%和5.7%(P<0.05)。钙染色分别为7.3%、4.7%和6.5%(P>0.05)。(2)慢性肝病、癌及癌旁、对照组免疫组化阳性率分别为:20.8%、16.3%、14.0%和0%(P>0.05)。部分肝癌和癌旁组织透射电镜观察发现纳米细菌样微生物结构(3/5)。结论部分慢性肝病、肝癌病人血清、组织中存在纳米细菌感染,慢性肝病血清中纳米细菌感染率高于健康人。
OBJECTIVE: To study the infection of the nosocomial bacteria (NB) in the serum and tissues of patients with chronic liver disease and primary hepatocellular carcinoma (HCC), and to provide new insights into their pathogenesis. Methods Serum samples of 55 patients with chronic liver disease (25 patients with chronic hepatitis B and 30 patients with posthepatitic cirrhosis), 43 patients with hepatocellular carcinoma and 336 healthy controls were examined by ELISA, immunohistochemistry and calcium staining. Fifty-three patients with chronic liver disease (28 patients with chronic hepatitis B and 25 Liver cirrhosis), 43 cases of liver cancer and 15 cases of control liver tissue immunohistochemical staining, some of the positive tissue transmission electron microscopy. Results (1) The positive rates of serum ELISA for chronic liver disease, liver cancer patients and healthy people were 20.0%, 9.3% and 8.0% respectively. The NB infection rate of chronic liver disease was higher than that of healthy people (P <0.05). The infection rates of immunohistochemical staining were 14.5%, 4.7% and 5.7% respectively (P <0.05). Calcium staining was 7.3%, 4.7% and 6.5%, respectively (P> 0.05). (2) The positive rates of immunohistochemistry in the control group were 20.8%, 16.3%, 14.0% and 0% respectively (P> 0.05). Part of liver cancer and adjacent tissues were observed by transmission electron microscopy of nanobacteria-like microbial structure (3/5). Conclusion Some of the patients with chronic liver disease and hepatocellular carcinoma have nosocomial bacterial infection in the serum and tissues, and the infection rate of nosocomial bacteria in chronic liver disease is higher than that in healthy people.